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14:50
jove.com
Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
39.0K Views. Morgridge Institute for Research. Here we describe a method for preparation of both single read and paired end Illumina mRNA-Seq sequencing libraries for gene expression analysis based on T7 linear RNA amplification. This protocol requires only 10 nanograms of starting total RNA and generates highly consistent libraries ...
39.2K views
Oct 27, 2011
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