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Ucallm Biology
How to Operate an ELISA Microplate for Absorbance Measurement
Measuring absorbance is the final, critical step in a colorimetric ELISA, determining the quantitative result. Proper operation ensures accuracy and reproducibility. Below is a concise guide to the standard operating procedure. I. Pre-Read Preparation Reaction Termination: Add the stop solution (e.g., sulfuric acid for TMB substrate) as per ...
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