Figure 1: Enzymatic cascade of the pyrosequencing reaction in the example of a bisulfite-treated template sequence, including a CpG position that is methylated on approximately 50% of all molecules.

Multiple fields of microbiology rely on techniques of microbial identification, such as in food microbiology for detecting contaminants, and in microbial ecology to characterize biodiversity. Image ...

Pyrosequencing technique is based on sequencing-by-synthesis principle. The method is based on the sequencing of single stranded DNA and now-a-days sequencing of double stranded DNA is also possible.

DNA methylation is a form of epigenetics in which gene expression is encouraged or inhibited by the addition of a methyl group on cytosines. It is highly implicated in development, cell ...