With new upstream bioprocessing technologies continuing to increase yield and product quantities, the pressure for process improvement has fallen firmly on the downstream purification stage. The ...

The protocol describes a method for capture of secreted hexahistidine-tagged proteins using expanded-bed adsorption immobilized-metal affinity chromatography. The starting material for the procedure ...

Preparative cell separation is an important tool to provide a highly purified population of specific cell type for diagnostic, biotechnological and biomedical applications 1. However, challenges ...