Affinity chromatography is a separation process used to purify molecules or a group of molecules that are in a biochemical mixture. It employs two phases; a stationary phase and a mobile phase.

The protocol describes a method for capture of secreted hexahistidine-tagged proteins using expanded-bed adsorption immobilized-metal affinity chromatography. The starting material for the procedure ...

Purification by affinity chromatography on immobilized calmodulin is suitable for recombinant proteins expressed in bacteria because endogenous bacterial calmodulin-binding proteins are unknown.